The A27 has been reopened in both directions following a serious accident in the early hours of the morning.
Emergency services shut the road in both directions between Station Road (Drusilla's Roundabout, Alfriston) and A26 (Beddingham) for more than eight hours after the accident.
Officers were called at 1.26am to the incident near Middle Farm after a Renault Clio travelling westbound hit a Ford Focus travelling eastbound.
The 36-year-old driver of the Renault Clio, from Bexhill, suffered serious injuries and was taken by ambulance to the Royal Sussex County Hospital, Brighton.
The other driver, a 23-year-old man from South Ockendon, Essex, also suffered serious injuries and was taken to the Royal Sussex.
His passenger, a 26-year-old man from London, suffered minor injuries.
The road was closed in both directions for an investigations to take place and to clear debris and an oil spillage. Part of the road surface has also been damaged.
The road was reopened at 9.45am.
Firefighters from Lewes had to cut one of the drivers free.
Sergeant David Morgan said: "We are appealing for witnesses to this serious collision. I am particularly keen to speak to the driver of a white van who was travelling eastbound and flashed his headlights at another vehicle possibly warning him of the collision ahead.
"We also want to thank motorists for their patience while the road has been closed."
Anyone with information should contact police on 101 or email email@example.com quoting Operation Earlham.