The A27 has been closed in both directions at Firle, near Lewes.
The road was closed at around 6pm following a collision between a motorcyclist and a car.
The road has been closed in both the east and westbound directions while emergency services at the scene wait for accident investigators to arrive.
It is not yet known whether anyone was injured in the collision.
A spokeswoman for East Sussex Fire and Rescue said they had attended the crash at 5.42pm.
Vehicles at the scene were made safe by firefighters.
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