A train ploughed into 21 cows in calf which had wandered on to a railway track.
All the animals and their calves were killed despite the train driver's efforts to stop.
The animals are believed to have broken loose from a field belonging to Newhouse Farm, Sandhill Lane, Eridge, on the Kent and Sussex boundary.
Firefighters from Crowborough were called to the railway line after yesterday's accident at 5.15pm and remained until 11.30pm lifting the dead cattle away.
A crane brought in by Railtrack was also used.
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