The huge 5ft 5in high creation took volunteers a week to make.
Passers-by in Jubilee Square, Brighton, stopped to stare at the pompom, which was 12ft 6in wide and was put together as part of Brighton Fashion Week.
The idea was funded by a grant from the Royal Society for the Encouragement of Arts, Manufacturers and Commerce to promote the creative reuse of fashion and textiles.
The creation smashed the previous Guinness World Record, which measured 4ft high and 10ft 8in wide.
The pompom will now be taken to a recycling plant where it will be broken down into natural fibres and spun into a giant ball of yarn which will eventually be used to make socks and the insides of jackets.
Brighton Fashion Week takes place from June 1 to 5 and ends Brighton Festival Fringe.
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